Micafungin sodium (FK 463) is the sodium salt form of micafungin, a semi-synthetic echinocandin derived from a natural product of the fungus Coleophoma empetri with antifungal activity.

Micafungin (10 mg/mL) 在大多数分离株中从表型上减少了生物膜的形成。在micafungin处理的样品中，所有测试的基因的mRNA转录水平也显著降低[1]。Micafungin与KB425796-C的组合具有杀真菌作用，并显著减少了CFU数量，与单独使用每种化合物时在所有检查的时间点观察到的抑真菌作用（CFU无减少）形成鲜明对比[2]。

Micafungin (1 mg/kg) 与生理盐水处理的小鼠相比，显著延长存活时间。与仅使用micafungin (0.1 mg/kg) 处理的小鼠相比，联合使用micafungin (0.1 mg/kg)与KB425796-C (32 mg/kg) 的小鼠显示出存活时间有延长的趋势。micafungin处理的小鼠肝脏中CFU数量减少，尽管其清除效果不如在肾脏中观察到的那样显著。micafungin与KB425796-C的联合治疗与仅使用micafungin治疗相比，在所有检测的剂量下都显著减少了CFU数量。与AMPH治疗的动物相比，KB425796-C联合micafungin的清除效果更为显着[2]。

HEK-GPR119 cells are transfected with GloSensor 22F plasmid and used for dynamic cAMP measurements 24-30 h later. Cell suspensions are made by dislodging the cells using PBS wash and Accutase treatment followed by resuspension in culture media. Cells are then washed twice by pelleting through centrifugation (300 g, 5 min) and resuspension in assay buffer (Hank's Balanced Salt Solution supplemented with 20 mM HEPES and 0.01% fatty acid free BSA, pH 7.4). Cells are then counted and diluted to 600,000 cells/mL in buffer, before GloSensor cAMP reagent is added (2% v/v) and equilibrated with the cells for 2 h at 20°C with periodic mixing. 50 μl/well of cells are added to white-bottomed 384 well plates (30,000 cells/well) in triplicate and baseline luminescence is measuring using an Envision plate-reader. 5 μL of MBX-2982 (serially diluted in DMSO and then diluted 1:100 in assay buffer to obtain ×10 concentrated solution) is manually added to the assay wells to achieve the stated final concentration. Plates are incubated at 20°C with luminescence read at regular intervals to detect dynamic cAMP changes over time within the same wells. cAMP responses at each time-point are expressed as fold over control (vehicle-treated cells)[1].

Each fungal isolate is incubated statically in yeast-maltose (YM) agar broth for 24?h at 30°C.?Cryptococcus neoformans?YC203 is grown in YM broth medium for 20?h at 30°C with shaking at 200?r.p.m. A cell suspension is prepared by washing the cultured cells once with sterile saline.?A. fumigatus?FP1305 is cultured on a potato dextrose agar (PDA) slant for 4 days, and spores are then harvested in sterile saline and collected by filtering through gauze. Antifungal activity against all isolates, with the exception of C. neoformans, is measured by the micro-broth dilution method in 96-well culture plates using RPMI 1640 medium supplemented with?l-glutamine, but without sodium bicarbonate, and buffered to pH 7.0 with 0.165?m?MOPS. ForC. neoformans, yeast nitrogen base-glucose (YNBD) medium is used. For the assay, the test microorganism is inoculated into each well to yield 1×105?CFU/well, and the plates are then incubated for 20?h or 48?h at 37°C. Two end points are determined by microscopic observation: MEC, which is defined as a substantial reduction in fungal growth, and MIC, which is defined as a complete inhibition of growth.